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fluorescent qpcr instrument stepone plustm  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fluorescent qpcr instrument stepone plustm
    Fluorescent Qpcr Instrument Stepone Plustm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stepone+plustm+qpcr+instrument/fluorescent+qpcr+instrument+stepone+plustm/pm39626448-122-7-12
    Average 90 stars, based on 1 article reviews
    fluorescent qpcr instrument stepone plustm - by Bioz Stars, 2026-10
    90/100 stars

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    Polymerase Chain Reaction:

    Article Title: Oncogenic mutations in the DNA-binding domain of FOXO1 that disrupt folding: quantitative insights from experiments and molecular simulations
    Article Snippet: Thermal Shift Assay (TSA) FKH protein was exchanged into 20 mM Tris-HCl pH 8.0, 50 mM KCl, 5% w/v glycerol, 2 mM DTT, 0.2 mM EDTA, 2 mM MgCl 2 , at 5 μM concentration and 2X Sypro Orange (Thermo Fisher). .. Aliquots of 50 μl were loaded into PCR strips and analyzed on a StepOne PlusTM qPCR instrument (Thermofisher) with temperature gradient 25–95 °C ramp, 1 °C increment, 1 min hold/increment. .. Data from the ROX channel was baseline corrected and fit to a 2-state model in Matlab.

    Real-time Polymerase Chain Reaction:

    Article Title: Oncogenic mutations in the DNA-binding domain of FOXO1 that disrupt folding: quantitative insights from experiments and molecular simulations
    Article Snippet: Thermal Shift Assay (TSA) FKH protein was exchanged into 20 mM Tris-HCl pH 8.0, 50 mM KCl, 5% w/v glycerol, 2 mM DTT, 0.2 mM EDTA, 2 mM MgCl 2 , at 5 μM concentration and 2X Sypro Orange (Thermo Fisher). .. Aliquots of 50 μl were loaded into PCR strips and analyzed on a StepOne PlusTM qPCR instrument (Thermofisher) with temperature gradient 25–95 °C ramp, 1 °C increment, 1 min hold/increment. .. Data from the ROX channel was baseline corrected and fit to a 2-state model in Matlab.



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